3D Bio-nanofibrous Mats as Platforms for Cell Culturing

نویسندگان

  • Yong Liu
  • Xiao Liu
  • Jun Chen
  • Kerry J. Gilmore
  • Gordon G. Wallace
چکیده

Iron(III) ρ-toluenesulfonate hexahydrate, tetrahydrofuran formaldehyde, Na2HPO4, NaH2PO4, and NaCl were all obtained from Sigma-Aldrich. Poly(styrene-β-isobutylene-β-styrene) (SIBS) was supplied by Boston Scientific. Pyrrole monomer from Merck was distilled and stored at -18C before use. Gold coated mylar was from CP Films Inc. Rat PC12 cells were obtained from American Type Tissue Collection. Eagle’s minimal essential medium (DMEM), glutamine and horse serum were purchased from GIBCO. Fetal bovine serum (FBS) and collagen was provided by Thermoelectron and Purecol respectively. Nerve growth factor (NGF) and lexa Fluor 488 phalloidin were obtained by Invitrogen.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Preparation of antibacterial electrospun Poly lactic-co–glycolic acid nanofibers containing Hypericum Perforatum with bedsore healing property and evaluation of its drug release performance

Skin drug delivery systems with controlled release are suitable means for the local transfer of pharmaceutical compounds to the damaged and healthy layers of skin. Nanofibrous membrane prepares uniform moisture in the wound environment with less accumulation of fluid secretion due to its variable pore size. Electrospinning takes advantage of using herbal extracts in the form of electrospun nano...

متن کامل

Preparation of antibacterial electrospun Poly lactic-co–glycolic acid nanofibers containing Hypericum Perforatum with bedsore healing property and evaluation of its drug release performance

Skin drug delivery systems with controlled release are suitable means for the local transfer of pharmaceutical compounds to the damaged and healthy layers of skin. Nanofibrous membrane prepares uniform moisture in the wound environment with less accumulation of fluid secretion due to its variable pore size. Electrospinning takes advantage of using herbal extracts in the form of electrospun nano...

متن کامل

Synthesis of three-dimensional calcium carbonate nanofibrous structure from eggshell using femtosecond laser ablation

BACKGROUND Natural biomaterials from bone-like minerals derived from avian eggshells have been considered as promising bone substitutes owing to their biodegradability, abundance, and lower price in comparison with synthetic biomaterials. However, cell adhesion to bulk biomaterials is poor and surface modifications are required to improve biomaterial-cell interaction. Three-dimensional (3D) nan...

متن کامل

Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold.

The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering. Glutaraldehyde was used to treat the scaffolds water insoluble as well as improve their biostability for possible use in biomedical applications. Electrospinning of ...

متن کامل

Fabrication and Characterization of Thermoresponsive Polystyrene Nanofibrous Mats for Cultured Cell Recovery

Rapid cell growth and rapid recovery of intact cultured cells are an invaluable technique to maintain the biological functions and viability of cells. To achieve this goal, thermoresponsive polystyrene (PS) nanofibrous mat was fabricated by electrospinning of PS solution, followed by the graft polymerization of thermoresponsive poly(N-isopropylacrylamide)(PIPAAm) on PS nanofibrous mats. Image a...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2008